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megashortscript t7 transcription kit thermofisher scientific am1354  (Thermo Fisher)


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    Thermo Fisher megashortscript t7 transcription kit thermofisher scientific am1354
    Megashortscript T7 Transcription Kit Thermofisher Scientific Am1354, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/megashortscript+t7+transcription+kit+am1354/megashortscript+t7+transcription+kit/pm40579489-281-6-10
    Average 90 stars, based on 1 article reviews
    megashortscript t7 transcription kit thermofisher scientific am1354 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Tissue-targeted CRISPR-Cas9-mediated genome editing of multiple homeologs in F 0 generation Xenopus laevis embryos
    Article Snippet: Modified Barth’s saline (1X) (MBS) or Marc’s modified Ringers (MMR) (1X, without EDTA) MEGAshortscript T7 Transcription Kit (Thermofisher AM1354) Nuclease-free (or DEPC-treated) H 2 O Oligonucleotides Forward and reverse primer to amplify edited DNA for sequencing (10 mM; see Step 17) Target-specific forward primer for sgRNA template synthesis (10 mM; see Step 4) Universal reverse primer for sgRNA template synthesis (10 mM; see Step 5) Phenol red (25 μM) (optional; see Step 16) RNA loading dye (Ambion AM8546G) Sodium hydroxide (50 mM) TAE or TBE buffer Taq (5000 units/mL) and 10X buffer (e.g., NEB M0273) Tris (1 M, pH 8) Xenopus laevis embryos obtained using standard protocols ( Sive et al., 2000 )

    Article Title: Reduced female fertility due to sequestration of RNA Pol II by pervasive transcription in exosome RNase-depleted oocytes.
    Article Snippet: Each guide RNA (gRNA) was synthesized by in vitro transcription using aMEGAshortscript T7 Transcription Kit (ThermoFisher AM1354) and purified by a MEGAclear Transcription Clean-Up Kit (ThermoFisher AM1908).

    CRISPR:

    Article Title: SEC24C deficiency causes trafficking and glycosylation abnormalities in an epileptic encephalopathy with cataracts and dyserythropoeisis
    Article Snippet: .. CRISPR/Cas9 target sites in the zebrafish sec24C gene were identified using the CHOPCHOP web tool ( ). sgRNA templates were designed as previously described ( ). gRNAs were synthesized with the MEGAshortscript T7 transcription kit (AM1354, Thermo Fisher Scientific). .. One-cell–stage wild-type embryos were injected with 500 pg of purified Cas9 protein (CP01, PNA Bio Inc.) and 300 pg sgRNA.

    Article Title: SEC24C deficiency causes trafficking and glycosylation abnormalities in an epileptic encephalopathy with cataracts and dyserythropoeisis.
    Article Snippet: .. CRISPR/Cas9 genome editing CRISPR/Cas9 target sites in the zebrafish sec24C gene were identified using the CHOPCHOP web tool (26). sgRNA templates were designed as previously described (27). gRNAs were synthesized with the MEGAshortscript T7 transcription kit (AM1354, ThermoFisher Scientific; Waltham, MA). .. One-cell stage wild type embryos were injected with 500 pg of purified Cas9 protein (CP01, PNA Bio Inc.; Newbury Park, CA) and 300 pg sgRNA.

    Synthesized:

    Article Title: SEC24C deficiency causes trafficking and glycosylation abnormalities in an epileptic encephalopathy with cataracts and dyserythropoeisis
    Article Snippet: .. CRISPR/Cas9 target sites in the zebrafish sec24C gene were identified using the CHOPCHOP web tool ( ). sgRNA templates were designed as previously described ( ). gRNAs were synthesized with the MEGAshortscript T7 transcription kit (AM1354, Thermo Fisher Scientific). .. One-cell–stage wild-type embryos were injected with 500 pg of purified Cas9 protein (CP01, PNA Bio Inc.) and 300 pg sgRNA.

    Article Title: SEC24C deficiency causes trafficking and glycosylation abnormalities in an epileptic encephalopathy with cataracts and dyserythropoeisis.
    Article Snippet: .. CRISPR/Cas9 genome editing CRISPR/Cas9 target sites in the zebrafish sec24C gene were identified using the CHOPCHOP web tool (26). sgRNA templates were designed as previously described (27). gRNAs were synthesized with the MEGAshortscript T7 transcription kit (AM1354, ThermoFisher Scientific; Waltham, MA). .. One-cell stage wild type embryos were injected with 500 pg of purified Cas9 protein (CP01, PNA Bio Inc.; Newbury Park, CA) and 300 pg sgRNA.

    Article Title: The sex-dependent impact of PER2 polymorphism on sleep and activity in a novel mouse model of cranial-irradiation-induced hypersomnolence.
    Article Snippet: .. A specific sgRNA was synthesized in vitro using the MEGAshortscript T7 transcription kit (Thermo Fisher Scientific; AM1354) and purified with the MEGAclear kit (Thermo Fisher Scientific; AM1908). .. The sgRNA (50 ng/ μl), the Cas9 mRNA (100 ng/μL, TriLink Biotechnologies; Importance of Study Primary brain tumor patients experience a variety of cranial radiation therapy effects that diminish their quality of life.

    Produced:


    Purification:


    Article Title: The eutherian-specific histone H3.4 promotes germ cell development and reproductive fitness
    Article Snippet: .. A T7 promoter was added, and in vitro transcription was performed using the MEGAshortscript T7 Transcription Kit (AM1354, Thermo Fisher Scientific). sgRNAs were purified using the MEGAclear Transcription Clean-Up Kit (AM1908, Thermo Fisher Scientific). (2) The EnGen sgRNA Synthesis Kit (E3322S, NEB) was used according to the manufacturer’s instructions. .. In short, the EnGen sgRNA Template Oligo Designer ( https://nebiocalculator.neb.com/#!/sgrna , NEB) was used to design oligos for in vitro transcription of sgRNAs.

    Article Title: The sex-dependent impact of PER2 polymorphism on sleep and activity in a novel mouse model of cranial-irradiation-induced hypersomnolence.
    Article Snippet: .. A specific sgRNA was synthesized in vitro using the MEGAshortscript T7 transcription kit (Thermo Fisher Scientific; AM1354) and purified with the MEGAclear kit (Thermo Fisher Scientific; AM1908). .. The sgRNA (50 ng/ μl), the Cas9 mRNA (100 ng/μL, TriLink Biotechnologies; Importance of Study Primary brain tumor patients experience a variety of cranial radiation therapy effects that diminish their quality of life.

    In Vitro:

    Article Title: The eutherian-specific histone H3.4 promotes germ cell development and reproductive fitness
    Article Snippet: .. A T7 promoter was added, and in vitro transcription was performed using the MEGAshortscript T7 Transcription Kit (AM1354, Thermo Fisher Scientific). sgRNAs were purified using the MEGAclear Transcription Clean-Up Kit (AM1908, Thermo Fisher Scientific). (2) The EnGen sgRNA Synthesis Kit (E3322S, NEB) was used according to the manufacturer’s instructions. .. In short, the EnGen sgRNA Template Oligo Designer ( https://nebiocalculator.neb.com/#!/sgrna , NEB) was used to design oligos for in vitro transcription of sgRNAs.

    Article Title: The sex-dependent impact of PER2 polymorphism on sleep and activity in a novel mouse model of cranial-irradiation-induced hypersomnolence.
    Article Snippet: .. A specific sgRNA was synthesized in vitro using the MEGAshortscript T7 transcription kit (Thermo Fisher Scientific; AM1354) and purified with the MEGAclear kit (Thermo Fisher Scientific; AM1908). .. The sgRNA (50 ng/ μl), the Cas9 mRNA (100 ng/μL, TriLink Biotechnologies; Importance of Study Primary brain tumor patients experience a variety of cranial radiation therapy effects that diminish their quality of life.



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